WebThe transgene consists of a tamoxifen-inducible cre recombinase driven by the 2.3-kb mouse Col1a1, collagen, type I, alpha 1, promoter. The transgene insert contains a fusion product involving cre recombinase and a mutant form of the mouse estrogen receptor ligand binding domain. The mutant mouse estrogen receptor does not bind natural ligand ... WebBbs10 fl/fl mice with a Deleter-Cre mouse line. Following Cre-mediated excision of the Bbs10 allele, the Cre allele was removed through breeding the resulting heterozy-gous Bbs10 mice Bbs10+/−. The Bbs10+/− mice were then used for breeding to generate the total Bbs10 knockout mice Bbs10 −/, and were subsequently genotyped by PCR (Fig. 1b).
Enhancer of zeste homolog 2 promotes renal fibrosis after acute …
WebTo address this issue, we generated kidney-specific Sav1-knockout (Cdh16-Cre;Sav1(fl/fl) ) mice. Sav1 deficiency enhanced the proliferation of renal tubular epithelial cells in Cdh16 … WebMutation details: The Ksp-cadherin (Cdh16) promoter was used to drive the expression of a cre/ERT2 gene fusion in adult tubular epithelial cells in the kidney. Several lines were … florian nrw
LncRNA IRAR regulates chemokines production in tubular ... - Nature
Web003328 129S/Sv-Tg(Prm-cre)58Og/J A cre recombinase coding sequence, driven by the mouse protamine 1 promoter ( Prm ) in this transgenic strain, mediates the efficient recombination of a cre target transgene in the male germ line, but not in other tissues. The use of embryonic stem cells of this strain can substantially simplify the production of … WebB–D) and crossbreeding with CDH16-Cre transgenic mice. How-ever, kidney-targeted Fnip2 inactivation also did not cause any phenotype in mouse kidney (Fig. 1E). Indeed, we could not find any phenotype in whole-body Fnip2 knockout mice that affected life span. The Relative Expression Levels of Fnip1 and Fnip2 Differ from Organ to Organ. WebEZH2 conditional knockout mice were generated by breeding our EZH2 fl/fl mice with Cdh16-Cre mice to obtain Cdh16-Cre +: EZH2 fl/fl mice (EZH2-cKO) and Cdh16-Cre −: EZH2 fl/fl mice (EZH2-WT). Our data showed that EZH2-cKO or inhibition of EZH2 with 3-DZNep significantly alleviated kidney damage and reduced serum creatinine and BUN in … florian obermeyer zahnarzt